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Issue 1, 2016
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A knot polymer mediated non-viral gene transfection for skin cells

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Abstract

A knot polymer, poly[bis(2-acryloyl)oxyethyl disulphide-co-2-(dimethylamino) ethyl methacrylate] (DSP), was synthesized, optimized and evaluated as a non-viral vector for gene transfection for skin cells, keratinocytes. With recessive dystrophic epidermolysis bullosa keratinocytes (RDEBK-TA4), the DSP exhibited high transfection efficacy with both Gaussia luciferase marker DNA and the full length COL7A1 transcript encoding the therapeutic type VII collagen protein (C7). The effective restoration of C7 in C7 null-RDEB skin cells indicates that DSP is promising for non-viral gene therapy of recessive dystrophic epidermolysis bullosa (RDEB).

Graphical abstract: A knot polymer mediated non-viral gene transfection for skin cells

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Supplementary files

Article information


Submitted
30 Jun 2015
Accepted
31 Aug 2015
First published
15 Sep 2015

Biomater. Sci., 2016,4, 92-95
Article type
Communication
Author version available

A knot polymer mediated non-viral gene transfection for skin cells

L. Cutlar, Y. Gao, A. Aied, U. Greiser, E. M. Murauer, D. Zhou and W. Wang, Biomater. Sci., 2016, 4, 92
DOI: 10.1039/C5BM00216H

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