Issue 28, 2016

Quantitative analysis of vitamin K1 in fruits and vegetables by isotope dilution LC-MS/MS

Abstract

A method has been developed for quantitative analysis of vitamin K1 in vegetables and fruits by LC-MS/MS. The method employed a neutral aluminum oxide column for sample clean-up. Considering matrix effects, an isotope standard (vitamin K1-D7) was added to correct matrix interferences, as well as pretreatment losses and instrument variability. Spiked recoveries in model matrices were 88.3–90.1% for spinach, 84–110% for lotus roots, and 108–115.6% for grapes. The relative standard deviations (RSDs) were 1.0–7.1% for intra-day and 2.8–8.8% for inter-day. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001 mg kg−1 and 0.004 mg kg−1, respectively. The method is sensitive and reliable, which is successfully applied to different vegetable and fruit matrices. Vitamin K1 in fruit samples ranged from 0.008 mg kg−1 to 0.401 mg kg−1, and in vegetable samples from 0.0032 mg kg−1 to 3.973 mg kg−1.

Graphical abstract: Quantitative analysis of vitamin K1 in fruits and vegetables by isotope dilution LC-MS/MS

Article information

Article type
Paper
Submitted
12 May 2016
Accepted
17 Jun 2016
First published
23 Jun 2016

Anal. Methods, 2016,8, 5707-5711

Quantitative analysis of vitamin K1 in fruits and vegetables by isotope dilution LC-MS/MS

B. Huang, Z. Wang, J. Yao, X. Ke, J. Xu, X. Pan, X. Xu, M. Lu and Y. Ren, Anal. Methods, 2016, 8, 5707 DOI: 10.1039/C6AY01324D

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