Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry

Abstract

The interaction between the Homer EVH domain and RyR plays an important role in calcium signaling channels in the heart and the skeletal muscles. A biosensor based on imaging ellipsometry was used to analyze the binding affinity between the Homer EVH domain and RyR. The results confirm that the Homer EVH domain can recognize RyR specifically with high binding affinity of approximately 10⁻⁸ M. This information will contribute to understanding of the role of the interaction between the Homer EVH domain and RyR in intracellular calcium channels.