Jump to main content
Jump to site search

Issue 5, 2016
Previous Article Next Article

Enantioseparation of fourteen amino alcohols by nonaqueous capillary electrophoresis using lactobionic acid/d-(+)-xylose–boric acid complexes as chiral selectors

Author affiliations

Abstract

An interesting study on chiral nonaqueous capillary electrophoresis (NACE) was developed in this paper. Two new chiral selectors, a lactobionic acid–boric acid complex and a D-(+)-xylose–boric acid complex were respectively in situ synthesized in nonaqueous background electrolytes (BGEs) containing methanol and triethylamine. They were found to be applicable for the enantioseparation of fourteen amino alcohols including eight β-blockers and six β-agonists by NACE. In order to achieve good enantioseparation, the effects of chiral selector concentration, BGE composition, capillary temperature, and applied voltage were systematically investigated. Under the optimized conditions, most of the tested amino alcohols achieved good chiral resolution. The effects of the molecular structures of chiral selectors and analytes on enantioseparation were discussed in terms of molecular interactions. The method was proved to be suitable for routine analysis of propranolol enantiomers, since it provided satisfactory results during linearity, precision, and accuracy (recovery) studies using the lactobionic acid–boric acid complex as the chiral selector. Good linearity was obtained in the range of 1.0–100.0 μg mL−1 for each propranolol enantiomer and the recoveries for them ranged from 96.4% to 105.9% with the relative standard deviation (RSD) less than 6.4%. This chiral NACE method was applied for the determination of propranolol enantiomers from tablets and the average contents were 101.3% for (R)-propranolol and 98.5% for (S)-propranolol (n = 5).

Graphical abstract: Enantioseparation of fourteen amino alcohols by nonaqueous capillary electrophoresis using lactobionic acid/d-(+)-xylose–boric acid complexes as chiral selectors

Back to tab navigation

Article information


Submitted
10 Oct 2015
Accepted
02 Jan 2016
First published
05 Jan 2016

Anal. Methods, 2016,8, 1127-1134
Article type
Paper
Author version available

Enantioseparation of fourteen amino alcohols by nonaqueous capillary electrophoresis using lactobionic acid/D-(+)-xylose–boric acid complexes as chiral selectors

N. An, L. Wang, J. Zhao, L. Lv, N. Wang and H. Guo, Anal. Methods, 2016, 8, 1127 DOI: 10.1039/C5AY02686E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements