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Issue 7, 2016
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Rapid fabrication of functionalized plates for peptides, glycopeptides and protein purification and mass spectrometry analysis

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Abstract

A rapid and simple approach for fabricating a disposable functionalized membrane on matrix-assisted laser desorption ionization (MALDI) targets, glass, or plastic substrates, without using complex mechanical protocols or chemical reactions, was developed for sample enrichment and mass spectrometry analysis. By coating functionalized-silica particles on a polydimethylsiloxane (PDMS)-coated plate, these particles can form a monolayer of materials on the PDMS membrane for sample handling without peeling off. An octadecyl(C18)-functionalized plate was fabricated by coating porous C18-silica particles on a PDMS-coated plate. The C18 particle-coated PDMS plate (CP plate) has better sensitivity than C18 tips and magnetic nanoparticles, along with a higher sample recovery (64.3 ± 4.9%) compared to the C18 tip method, when analyzing trace amounts of 5 fm BSA digest samples. The CP plate shows significantly higher urea/SDS removal efficiency on the cell lysate proteome compared to C18 tips. The capacity of the C18 spot (∼2.8 mm in diameter) on the CP plate was ∼10 μg of BSA digests. A hydrophilic particle-coated PDMS plate was also fabricated and successfully used for glycopeptide enrichment and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis.

Graphical abstract: Rapid fabrication of functionalized plates for peptides, glycopeptides and protein purification and mass spectrometry analysis

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Publication details

The article was received on 17 Jan 2016, accepted on 26 Feb 2016 and first published on 26 Feb 2016


Article type: Paper
DOI: 10.1039/C6AN00113K
Analyst, 2016,141, 2183-2190

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    Rapid fabrication of functionalized plates for peptides, glycopeptides and protein purification and mass spectrometry analysis

    H. Liao, F. Tsai, C. Lai, M. Tseng, C. Y. Hsu and C. Chen, Analyst, 2016, 141, 2183
    DOI: 10.1039/C6AN00113K

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