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Issue 17, 2016
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Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors

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Abstract

Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning.

Graphical abstract: Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors

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Article information


Submitted
04 May 2016
Accepted
01 Jun 2016
First published
16 Jun 2016

This article is Open Access

Analyst, 2016,141, 5056-5061
Article type
Communication

Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors

V. L. Camus, G. Stewart, W. H. Nailon, D. B. McLaren and C. J. Campbell, Analyst, 2016, 141, 5056
DOI: 10.1039/C6AN01032F

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
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    [Original citation] - Published by the PCCP Owner Societies.
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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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