Issue 21, 2016
From the journal:

Analyst

Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes†

Saori Kamoto,a   Masumi Hyugaa  and  Teru Kato*a  

* Corresponding authors

a Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
E-mail: kato@stf.teu.ac.jp

Abstract

We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.

Graphical abstract: Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

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Article information

DOI
https://doi.org/10.1039/C6AN00912C
Article type
Paper
Submitted
19 Apr 2016
Accepted
07 Aug 2016
First published
08 Aug 2016

Analyst, 2016,141, 6087-6092

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Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

S. Kamoto, M. Hyuga and T. Kato, Analyst, 2016, 141, 6087 DOI: 10.1039/C6AN00912C

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