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Issue 20, 2016
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Super-resolution imaging for monitoring cytoskeleton dynamics

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The cytoskeleton is a key cellular structure that is important in the control of cellular movement, structure, and sensing. To successfully image the individual cytoskeleton components, high resolution and super-resolution fluorescence imaging methods are needed. This review covers the three basic cytoskeletal elements and the relative benefits and drawbacks of fixed versus live cell imaging before moving on to recent studies using high resolution and super-resolution techniques. The techniques covered include the near-diffraction limited imaging methods of confocal microscopy and TIRF microscopy and the super-resolution fluorescence imaging methods of STORM, PALM, and STED.

Graphical abstract: Super-resolution imaging for monitoring cytoskeleton dynamics

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The article was received on 29 Mar 2016, accepted on 10 Aug 2016 and first published on 10 Aug 2016

Article type: Critical Review
DOI: 10.1039/C6AN00731G
Citation: Analyst, 2016,141, 5674-5688
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    Super-resolution imaging for monitoring cytoskeleton dynamics

    S. A. Finkenstaedt-Quinn, T. A. Qiu, K. Shin and C. L. Haynes, Analyst, 2016, 141, 5674
    DOI: 10.1039/C6AN00731G

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