Issue 11, 2016

Supramolecular biosensors based on electropolymerised pyrrole–cyclodextrin modified surfaces for antibody detection

Abstract

The self-assembly of an adamantane-appended polymer bearing an antigen fragment on a polypyrrole–cyclodextrin modified surface provides a highly sensitive immunosensor with low limits of detection for celiac disease related targets. The pyrrole-carboxylic acid films were formed on the surface of gold electrodes by electropolymerisation and followed by covalent attachment of cyclodextrin units. Surface plasmon resonance measurements confirmed the role of the host/guest interactions between adamantane moieties and β-cyclodextrin hosts in the formation of the supramolecular sensor interface. Furthermore, this novel electrochemical supramolecular platform was effective in the amperometric detection of anti-gliadin antibodies in spiked serum samples with very good signal recovery.

Graphical abstract: Supramolecular biosensors based on electropolymerised pyrrole–cyclodextrin modified surfaces for antibody detection

Article information

Article type
Paper
Submitted
04 Mar 2016
Accepted
06 Apr 2016
First published
06 Apr 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 3274-3279

Supramolecular biosensors based on electropolymerised pyrrole–cyclodextrin modified surfaces for antibody detection

E. Wajs, N. Fernández and A. Fragoso, Analyst, 2016, 141, 3274 DOI: 10.1039/C6AN00532B

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