Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 10, 2016
Previous Article Next Article

A hydrogel based rapid test method for detection of Escherichia coli (E. coli) in contaminated water samples

Author affiliations

Abstract

We have formulated a new chemical composition for rapid detection of Escherichia coli (E. coli) with currently available enzymatic substrates. We have evaluated the performance of the new chemical composition with different kinds of bacteria, and metallic and ionic interferences and optimized the chemical composition for rapid and specific detection of E. coli. We used a novel hydrogel based porous matrix to encapsulate the optimized chemical compounds and incorporated it within a readily available plunger-tube assembly. This overall system allows efficient, field deployable, rapid testing of water samples by simultaneously pre-concentrating and detecting E. coli within one integrated unit. We were able to detect E. coli concentrations of 4 × 106 CFU mL−1 to 4 × 105 CFU mL−1 within 5 min and 4 × 104 CFU mL−1 to 400 CFU mL−1 within 60 min using the integrated plunger-tube assembly containing the hydrogel matrix.

Graphical abstract: A hydrogel based rapid test method for detection of Escherichia coli (E. coli) in contaminated water samples

Back to tab navigation

Supplementary files

Article information


Submitted
17 Feb 2016
Accepted
20 Apr 2016
First published
21 Apr 2016

Analyst, 2016,141, 2920-2929
Article type
Paper

A hydrogel based rapid test method for detection of Escherichia coli (E. coli) in contaminated water samples

N. S. K. Gunda, R. Chavali and S. K. Mitra, Analyst, 2016, 141, 2920
DOI: 10.1039/C6AN00400H

Social activity

Search articles by author

Spotlight

Advertisements