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Issue 46, 2015
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Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

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Abstract

Progress in biofabrication technologies is mainly hampered by the limited number of suitable hydrogels that can act as bioinks. Here, we present a new bioink for 3D-printing, capable of forming large, highly defined constructs. Hydrogel formulations consisted of a thermoresponsive polymer mixed with a poly(ethylene glycol) (PEG) or a hyaluronic acid (HA) cross-linker with a total polymer concentration of 11.3 and 9.1 wt% respectively. These polymer solutions were partially cross-linked before plotting by a chemoselective reaction called oxo-ester mediated native chemical ligation, yielding printable formulations. Deposition on a heated plate of 37 °C resulted in the stabilization of the construct due to the thermosensitive nature of the hydrogel. Subsequently, further chemical cross-linking of the hydrogel precursors proceeded after extrusion to form mechanically stable hydrogels that exhibited a storage modulus of 9 kPa after 3 hours. Flow and elastic properties of the polymer solutions and hydrogels were analyzed under similar conditions to those used during the 3D-printing process. These experiments showed the ability to extrude the hydrogels, as well as their rapid recovery after applied shear forces. Hydrogels were printed in grid-like structures, hollow cones and a model representing a femoral condyle, with a porosity of 48 ± 2%. Furthermore, an N-hydroxysuccinimide functionalized thermoplastic poly-ε-caprolactone (PCL) derivative was successfully synthesized and 3D-printed. We demonstrated that covalent grafting of the developed hydrogel to the thermoplastic reinforced network resulted in improved mechanical properties and yielded high construct integrity. Reinforced constructs also containing hyaluronic acid showed high cell viability of chondrocytes, underlining their potential for further use in regenerative medicine applications.

Graphical abstract: Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

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Supplementary files

Publication details

The article was received on 12 Aug 2015, accepted on 07 Oct 2015 and first published on 09 Oct 2015


Article type: Paper
DOI: 10.1039/C5TB01645B
Citation: J. Mater. Chem. B, 2015,3, 9067-9078

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    Biofabrication of reinforced 3D-scaffolds using two-component hydrogels

    K. W. M. Boere, M. M. Blokzijl, J. Visser, J. E. A. Linssen, J. Malda, W. E. Hennink and T. Vermonden, J. Mater. Chem. B, 2015, 3, 9067
    DOI: 10.1039/C5TB01645B

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