Issue 34, 2015

A novel organic–inorganic hybrid polyoxometalate for the selective adsorption/isolation of β-lactoglobulin

Abstract

A novel polyoxometalate (POM)-based organic–inorganic hybrid [C33H24O4]H3PMo12O40, namely, TPPA–PMo12, is prepared via a one-pot hydrothermal reaction between a Keggin POM (H3PMo12O40, PMo12) and a star-like N-donor ligand (tri(4-pyridylphenyl)amine, TPPA). The hybrid polyoxometalate is confirmed by characterization with XRD, FT-IR, TGA, SEM and EDS. It exhibits excellent adsorption performance towards β-lactoglobulin, and thus a solid-phase extraction procedure was established for the efficient and selective isolation of β-lactoglobulin from complex sample matrices. At pH 5.0, an adsorption efficiency of 99.2% is achieved for processing 100 μg mL−1 β-lactoglobulin in 1.0 mL aqueous solution with 0.5 mg TPPA–PMo12 as an adsorbent. The adsorption behavior of β-lactoglobulin fits the Langmuir model, corresponding to a theoretical adsorption capacity of 1428 mg g−1. The retained β-lactoglobulin could be readily recovered by rinsing with 0.05 mol L−1 Tris-HCl buffer, facilitating a recovery of 91.5%. The hybrid polyoxometalate was practically applied to the selective isolation of β-lactoglobulin from milk whey, and SDS-PAGE assay results clearly indicate that β-lactoglobulin of high-purity is obtained.

Graphical abstract: A novel organic–inorganic hybrid polyoxometalate for the selective adsorption/isolation of β-lactoglobulin

Supplementary files

Article information

Article type
Paper
Submitted
01 Jul 2015
Accepted
28 Jul 2015
First published
28 Jul 2015

J. Mater. Chem. B, 2015,3, 6964-6970

Author version available

A novel organic–inorganic hybrid polyoxometalate for the selective adsorption/isolation of β-lactoglobulin

Q. Chen, D. Zhang, M. Wang, X. Chen and J. Wang, J. Mater. Chem. B, 2015, 3, 6964 DOI: 10.1039/C5TB01298H

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