Issue 1, 2015

Tri-peptide cationic lipids for gene delivery

Abstract

Several novel tri-peptide cationic lipids were designed and synthesized for delivering DNA and siRNA. They have tri-lysine and tri-ornithine as headgroups, a carbamate group as a linker and 12 and 14 carbon atom alkyl groups as tails. These tri-peptide cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. Their particle size, zeta potential and DNA-binding were characterized to show that they were suitable for gene transfection. Further results indicate that these lipids can transfer DNA and siRNA very efficiently into NCI-H460 and Hep-2 tumor cells. The selected lipid, CDO14, was able to deliver combined siRNAs against c-Myc and VEGF for silencing distinct oncogenic pathways in lung tumors of mice, with little in vitro and in vivo toxicity.

Graphical abstract: Tri-peptide cationic lipids for gene delivery

Supplementary files

Article information

Article type
Paper
Submitted
07 Aug 2014
Accepted
24 Sep 2014
First published
30 Sep 2014

J. Mater. Chem. B, 2015,3, 119-126

Author version available

Tri-peptide cationic lipids for gene delivery

Y. Zhao, S. Zhang, Y. Zhang, S. Cui, H. Chen, D. Zhi, Y. Zhen, S. Zhang and L. Huang, J. Mater. Chem. B, 2015, 3, 119 DOI: 10.1039/C4TB01312C

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