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Issue 7, 2015
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Graphene oxide single sheets as substrates for high resolution cryoTEM

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Abstract

CryoTEM is an important tool in the analysis of soft matter, where generally defocus conditions are used to enhance the contrast in the images, but this is at the expense of the maximum resolution that can be obtained. Here, we demonstrate the use of graphene oxide single sheets as support for the formation of 10 nm thin films for high resolution cryoTEM imaging, using DNA as an example. With this procedure, the overlap of objects in the vitrified film is avoided. Moreover, in these thin films less background scattering occurs and as a direct result, an increased contrast can be observed in the images. Hence, imaging closer to focus as compared with conventional cryoTEM procedures is achieved, without losing contrast. In addition, we demonstrate an ∼1.8 fold increase in resolution, which is crucial for accurate size analysis of nanostructures.

Graphical abstract: Graphene oxide single sheets as substrates for high resolution cryoTEM

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Publication details

The article was received on 22 Nov 2014, accepted on 09 Dec 2014 and first published on 10 Dec 2014


Article type: Paper
DOI: 10.1039/C4SM02587C
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Citation: Soft Matter, 2015,11, 1265-1270
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    Graphene oxide single sheets as substrates for high resolution cryoTEM

    M. W. P. van de Put, J. P. Patterson, P. H. H. Bomans, N. R. Wilson, H. Friedrich, R. A. T. M. van Benthem, G. de With, R. K. O'Reilly and N. A. J. M. Sommerdijk, Soft Matter, 2015, 11, 1265
    DOI: 10.1039/C4SM02587C

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