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Issue 11, 2015
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Insights into the deselenization of selenocysteine into alanine and serine

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Abstract

The development of native chemical ligation coupled with desulfurization has allowed ligation at several new ligation junctions. However, desulfurization also converts all cysteine residues in the protein sequence into alanine. Deselenization of selenocysteine, in contrast, selectively removes the selenol group to give alanine in the presence of unprotected cysteines. In this study we shed more light onto the deselenization mechanism of selenocysteine to alanine and provide optimized conditions for the reaction. The deselenization can be accomplished in one minute under anaerobic conditions to give alanine. Under aerobic conditions (oxygen saturation), selenocysteine is converted into serine.

Graphical abstract: Insights into the deselenization of selenocysteine into alanine and serine

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Supplementary files

Article information


Submitted
13 Jul 2015
Accepted
06 Aug 2015
First published
06 Aug 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 6207-6212
Article type
Edge Article
Author version available

Insights into the deselenization of selenocysteine into alanine and serine

S. Dery, P. S. Reddy, L. Dery, R. Mousa, R. N. Dardashti and N. Metanis, Chem. Sci., 2015, 6, 6207
DOI: 10.1039/C5SC02528A

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