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Issue 8, 2015
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Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

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Abstract

Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoactivation of individual cells in close spatial proximity.

Graphical abstract: Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

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Supplementary files

Article information


Submitted
02 May 2015
Accepted
09 Jun 2015
First published
17 Jun 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 4508-4512
Article type
Edge Article
Author version available

Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

M. N. Tran, R. F. Rarig and D. M. Chenoweth, Chem. Sci., 2015, 6, 4508
DOI: 10.1039/C5SC01601K

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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