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Issue 66, 2015
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Conformational structure-dependent molecular recognition of two aptamers for tetracycline

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Abstract

Different aptamers towards one target molecule can be selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX), however, not all aptamers have real world practicability. In this study, conformational structure-dependent molecular recognition of two aptamers towards tetracycline (TC), 76 mer and 40 mer, was studied both quantitatively and computationally. Two formats of competitive enzyme-linked aptamer assay (ELAA), a molecular docking module and Isothermal Titration Calorimeter (ITC) analysis were used to further investigate the two selected aptamers. With longer strand length, more G, C bases, and more recognition sites for TC, the 76 mer aptamer showed better performance than the 40 mer aptamer. Deciphering the relevance of aptamers with different molecular characteristics towards one target molecule can furnish as a referral guidance for aptamer selection and further practical application.

Graphical abstract: Conformational structure-dependent molecular recognition of two aptamers for tetracycline

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Publication details

The article was received on 01 May 2015, accepted on 10 Jun 2015 and first published on 11 Jun 2015


Article type: Communication
DOI: 10.1039/C5RA08050A
RSC Adv., 2015,5, 53796-53801

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    Conformational structure-dependent molecular recognition of two aptamers for tetracycline

    S. Wang, J. Liu, Y. Dong, H. Su and T. Tan, RSC Adv., 2015, 5, 53796
    DOI: 10.1039/C5RA08050A

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