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Issue 29, 2015
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Enzymatic multi-functionalization of microparticles under aqueous neutral conditions

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Abstract

The immobilization of proteins and enzymes on microparticles enables innovative biofunctionalization and facilitates the recycling of, for example, catalysts in a biocatalytic process. When enzymes are used for the immobilization of proteins to material surfaces, site-specificity can be achieved, and the immobilization reaction can proceed under mild reaction conditions in an aqueous environment. By selectively oxidizing the exposed tyrosyl side chains of protein to reactive o-quinones using molecular oxygen as electron acceptor, tyrosinase promotes the formation of new covalent bonds via 1,4-addition of nucleophilic moieties to the o-quinones. The introduction of tyrosinase-susceptible tyrosine residues (Y-tag) to a protein by genetic engineering can thus enable site-specific crosslinking and site-specific protein immobilization. In this study, several variants of the fluorescent protein GFPuv were produced in order to investigate the tyrosinase-mediated crosslinking and immobilization reaction. The Y-tag was a target for rapid protein–protein crosslinking by tyrosinase catalysis. Moreover, low concentrations of tyrosinase were sufficient to obtain detectable fluorescent microparticles using the Y-tagged GFPuv variants, e.g. 0.4 μM (50 μg ml−1) protein concentrations. Eventually, we showed that this enzyme-based technology allows for the multiple functionalization of microparticles using different fluorescent proteins.

Graphical abstract: Enzymatic multi-functionalization of microparticles under aqueous neutral conditions

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Publication details

The article was received on 12 Jan 2015, accepted on 13 Feb 2015 and first published on 16 Feb 2015


Article type: Paper
DOI: 10.1039/C5RA00669D
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Citation: RSC Adv., 2015,5, 22319-22325
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    Enzymatic multi-functionalization of microparticles under aqueous neutral conditions

    G. Faccio, S. Senkalla, L. Thöny-Meyer and M. Richter, RSC Adv., 2015, 5, 22319
    DOI: 10.1039/C5RA00669D

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