Deep eutectic solvents as a new class of draw agent to enrich low abundance DNA and proteins using forward osmosis

Abstract

The present study explores the potential of green and sustainable deep eutectic solvents (DESs) as an alternative energy source for concentrating proteins (>6 times) and DNA (>3 times) using forward osmosis (FO). The distinct colligative properties like high osmotic pressure (Π > 300 atm) and low water activity (a_w, ≤0.1) of the DESs make them an ideal draw solution in the FO process. These novel draw solutions in combination with a composite semi-permeable membrane were successfully tested for enriching biomacromolecules. Recovery of the draw agent was achieved by chilling the diluted solution after FO, which could then be reused in the process.