Investigating oxalate biosynthesis in the wood-decaying fungus Gloeophyllum trabeum using 13C metabolic flux analysis

Liangpeng Zhuang; Weihua Guo; Makoto Yoshida; Xueyang Feng; Barry Goodell

doi:10.1039/C5RA19203J

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Investigating oxalate biosynthesis in the wood-decaying fungus Gloeophyllum trabeum using ¹³C metabolic flux analysis†

Liangpeng Zhuang,‡^a Weihua Guo,‡^b Makoto Yoshida,^c Xueyang Feng*^b and Barry Goodell*^a

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* Corresponding authors

^a Department of Sustainable Biomaterials, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
E-mail: goodell@vt.edu

^b Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
E-mail: xueyang@vt.edu

^c Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Tokyo, JAPAN

Abstract

Oxalate synthesis was rigorously investigated in a wood-decaying fungus, Gloeophyllum trabeum, using ¹³C metabolic flux analysis, a method not previously explored in this type of system. Peroxisomal glyoxylate dehydrogenase and cytosolic oxaloacetate acetylhydrolase were found to contribute to the majority of oxalate synthesized under low and high nitrogen conditions, respectively.

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Article information

DOI: https://doi.org/10.1039/C5RA19203J
Article type: Communication
Submitted: 17 Sep 2015
Accepted: 18 Nov 2015
First published: 19 Nov 2015

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RSC Adv., 2015,5, 104043-104047

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Investigating oxalate biosynthesis in the wood-decaying fungus Gloeophyllum trabeum using ¹³C metabolic flux analysis

L. Zhuang, W. Guo, M. Yoshida, X. Feng and B. Goodell, RSC Adv., 2015, 5, 104043 DOI: 10.1039/C5RA19203J

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