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Issue 109, 2015
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Enhancement of thermal and pH stability of an alkaline metalloprotease by nano-hydroxyapatite and its potential applications

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Abstract

A purified calcium metalloprotease from Bacillus subtilis AKP(N) supplemented with hydroxyapatite nanoparticles [protease (+NP)] showed remarkable increase in thermostability (at 90 °C) over a broad alkaline pH range as compared to the non-supplemented enzyme. Thermodynamic analysis of the protease (+NP) showed decreases in Km and activation energy (Ea) with increased Vmax and deactivation energy (Ed) at both 40 °C and 90 °C. The protease (+NP) exhibited compatibility with various laboratory and commercial detergents (1%) at both 40 °C and 90 °C. The protease (+NP) was utilized to efficiently degrade feather to produce amino acids, removal of blood stains from cloth and tenderization of raw meat within a short time.

Graphical abstract: Enhancement of thermal and pH stability of an alkaline metalloprotease by nano-hydroxyapatite and its potential applications

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Publication details

The article was received on 11 Aug 2015, accepted on 08 Oct 2015 and first published on 20 Oct 2015


Article type: Paper
DOI: 10.1039/C5RA16179G
Citation: RSC Adv., 2015,5, 89346-89362
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    Enhancement of thermal and pH stability of an alkaline metalloprotease by nano-hydroxyapatite and its potential applications

    A. Mukhopadhyay and K. Chakrabarti, RSC Adv., 2015, 5, 89346
    DOI: 10.1039/C5RA16179G

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