Microparticle-enhanced production of ε-poly-l-lysine in fed-batch fermentation†
Abstract
In this study, the effects of talc microparticles on mycelia morphology and ε-poly-L-lysine (ε-PL) production by Streptomyces sp. M-Z18 were investigated for the first time. Changing mycelia morphology with talc microparticles has resulted in ε-PL production being enhanced. Talc microparticles caused a decrease of pellet diameter from 297.63 ± 156.33 to 205.65 ± 71.56 μm in shake-flask fermentation. Moreover, the maximum ε-PL production reached 2.51 ± 0.08 g L−1 in the presence of 10 g L−1 talc microparticles, which was increased by 50.30%, compared to the control (1.67 ± 0.11 g L−1). The scale-up processes were performed in batch and fed-batch fermentations in a 5 L fermenter with 10 g L−1 talc addition, and the final ε-PL production of 30.57 g L−1 was obtained at 168 h, with 44.06% increase over the control. Talc microparticles of 10 g L−1 were then supplied to the fed-batch fermentation with acidic pH shock and agro-industrial by-products, and ε-PL production was further increased to 62.36 g L−1 at 192 h, compared to 54.70 g L−1 of the control without supplementary talc. This is also the highest ε-PL production reported so far in the literature. These results suggested that talc addition could be an efficient and universal approach for enhancing ε-PL production in industry.