We introduced a simple, fast and label free fluorescent assay for uracil-DNA glycosylase activity based on the signal amplification of exonuclease I. This method exhibits a high sensitivity for UDG activity assay with a low detection limit of 0.0070 U mL−1.
Y. Ma, J. Zhao, X. Li, L. Zhang and S. Zhao, RSC Adv., 2015, 5, 80871 DOI: 10.1039/C5RA12958C
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