β-Amylase production from packaging-industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1

Abstract

Transport packaging factories generate a large amount of wastewater that contains residuals of starch glue. These residuals could be used as substrates for microorganism growth and enzyme production. In this study, β-amylase production using a new strain Paenibacillus chitinolyticus CKS1 was optimized using wastewater from a Serbian transport packaging factory. The optimization of β-amylase production was carried out using Response Surface Methodology (RSM). A Central Composite Design under the RSM with four interacting parameters (incubation time, inoculum concentration, casein hydrolysate concentration and yeast extract concentration) was employed to identify the optimal conditions for maximum β-amylase activity (334.20 U L⁻¹) as 62 h of incubation with 2.40% inoculum, 2.02 g L⁻¹ casein hydrolysate and 3.98 g L⁻¹ yeast extract. High performance liquid chromatography showed that the P. chitinolyticus CKS1 strain hydrolyzed starch to form maltose as a major product. Due to the application of wastewater as an inexpensive material for enzyme and maltose production it may be considered that the economic and eco-friendly aspects of this method are very promising.