Jump to main content
Jump to site search

Issue 78, 2015
Previous Article Next Article

Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

Author affiliations

Abstract

Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological tailoring has recently been achieved on CPCs by simply modulating the sizes of the initial particles. Herein, we aim to investigate the effects of nanotopological-tailored CPCs on the odontogenic differentiation of stem cells derived from human dental pulp (HDPSCs) as well as on their implicated signal pathways. The initial adhesion of the cells was substantially higher on nano-CPCs than on micro-CPCs. A series of indications of odontogenesis, including alkaline phosphatase activity and gene expressions (dentin matrix protein-1, dentin sialophosphoprotein, osteocalcin, ostepontin, and bone sialoprotein) were significantly stimulated on the nano-CPC in comparison to the micro-CPC. Furthermore, the integrin downstream pathways of the cells, including FAK, paxillin, Akt, MAPK, and NF-κB, were highly activated on the nano-CPC with respect to those on the micro-CPCs. Collectively, the nanotopological CPCs significantly enhance the odontogenic differentiation of HDPSCs when compared to conventional micro-CPCs through the integrin-associated signaling pathways, which implies that the nanotopological CPCs may be more potent in the repair and regeneration of dentin–pulp complex tissues.

Graphical abstract: Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

Back to tab navigation

Publication details

The article was received on 16 Jun 2015, accepted on 16 Jul 2015 and first published on 16 Jul 2015


Article type: Paper
DOI: 10.1039/C5RA11564G
Author version
available:
Download author version (PDF)
RSC Adv., 2015,5, 63363-63371

  •   Request permissions

    Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

    S. Lee, H. Yun, R. A. Perez, S. Gallinetti, M. Ginebra, S. Choi, E. Kim and H. Kim, RSC Adv., 2015, 5, 63363
    DOI: 10.1039/C5RA11564G

Search articles by author

Spotlight

Advertisements