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Issue 78, 2015
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Dihydroxycoumarins as highly selective fluorescent probes for the fast detection of 4-hydroxy-TEMPO in aqueous solution

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Abstract

Due to the biological significance of 4-hydroxy-TEMPO, its detection in biological systems such as blood serum is of great importance in both research and clinical applications. The following paper reports a novel fluorimetric method for the detection and quantitative determination of 4-hydroxy-TEMPO radicals in an aqueous solution using dihydroxycoumarins as fluorescent probes. Among the 17 coumarin derivatives studied, only some dihydroxycoumarins show high sensitivity, specificity and selectivity for 4-hydroxy-TEMPO. Among them, 6,7-dihydroxycoumarin (esculetin) exhibits the strongest fluorescence enhancement under the action of 4-hydroxy-TEMPO. In this assay, esculetin reacts with 4-hydroxy-TEMPO to exclusively yield a dimer. 6,7-Dihydroxycoumarin responds to 4-hydroxy-TEMPO quickly and shows a 2.5-fold fluorescence enhancement with an estimated detection limit of 285 μM.

Graphical abstract: Dihydroxycoumarins as highly selective fluorescent probes for the fast detection of 4-hydroxy-TEMPO in aqueous solution

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Article information


Submitted
22 Apr 2015
Accepted
17 Jul 2015
First published
21 Jul 2015

RSC Adv., 2015,5, 63807-63812
Article type
Paper

Dihydroxycoumarins as highly selective fluorescent probes for the fast detection of 4-hydroxy-TEMPO in aqueous solution

K. Żamojć, M. Zdrowowicz, W. Wiczk, D. Jacewicz and L. Chmurzyński, RSC Adv., 2015, 5, 63807
DOI: 10.1039/C5RA07283B

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