Issue 60, 2015

Industrial effluent as a substrate for glutaminase free l-asparaginase production from Pseudomonas plecoglossicida strain RS1; media optimization, enzyme purification and its characterization

Abstract

Glutaminase free L-asparaginase is a vital enzyme because of its anticancer potential. A potent bacterium isolated from a marine environment which produces glutaminase free L-asparaginase using M-9 medium with L-asparagine, was identified as Pseudomonas plecoglossicida RS1 by 16S rRNA gene sequencing. Statistical modeling was employed to optimize the medium using sugar cane industry effluent as the sole substrate for L-asparaginase production. The enzyme activity of L-asparaginase was higher with M-9 medium containing 0.8% effluent (3.25 ± 0.12 IU mL−1) compared to M-9 medium containing 0.3% L-asparagine (0.73 ± 0.08 IU mL−1). The apparent Km and Vmax of the purified L-asparaginase was 2.25 ± 0.61 mM and 8.9 ± 0.81 IU mL−1 min−1 respectively and the optimal activity of L-asparaginase was at pH 8.5 and 55 °C. This study highlights the use of industrial effluent as an alternate to L-asparagine for the production of L-asparaginase and how to improve the cost effectiveness of this enzyme.

Graphical abstract: Industrial effluent as a substrate for glutaminase free l-asparaginase production from Pseudomonas plecoglossicida strain RS1; media optimization, enzyme purification and its characterization

Supplementary files

Article information

Article type
Paper
Submitted
28 Mar 2015
Accepted
30 Apr 2015
First published
30 Apr 2015

RSC Adv., 2015,5, 48729-48738

Author version available

Industrial effluent as a substrate for glutaminase free L-asparaginase production from Pseudomonas plecoglossicida strain RS1; media optimization, enzyme purification and its characterization

G. Shakambari, B. M. Sumi, B. Ashokkumar, P. Palanivelu and P. Varalakshmi, RSC Adv., 2015, 5, 48729 DOI: 10.1039/C5RA05507E

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