A new and advanced CD4-binding reporter has been designed, produced and functionally assayed for the detection of T-helper cells. The sFv fragment fused N-terminally to the EGFP is a fluorescence probe successfully employed for counting T CD4+ cells using flow cytometry.
M. Ehsani, R. Emamzadeh, S. H. Zarkesh-Esfahani and M. Nazari, RSC Adv., 2015, 5, 56578 DOI: 10.1039/C5RA05333A
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