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Issue 54, 2015
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Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting

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Abstract

In the present work, gelatin/alginate and gelatin/alginate/hydroxyapatite (HAP) composite scaffolds were fabricated by 3D plotting based on high concentration gelatin/alginate pastes. At temperatures of 37 °C or above, the developed pastes could be easily processed into designed 3D structures; sequential crosslinking with Ca2+ ions (effective for alginate) and the carbodiimide EDC (effective for gelatin) resulted in stable scaffolds. Mechanical testing demonstrated that the plotted composite scaffolds had a significantly higher strength and modulus compared to most reported gelatin scaffolds prepared by conventional methods. Cell experiments with human bone marrow-derived mesenchymal stem cells (hBMSC) revealed that the gelatin/alginate composite scaffolds favor cell adhesion and support proliferation. Furthermore, the cells showed a homogeneous distribution and excellent migration in the inner regions of the plotted composite scaffolds over 21 days. In conclusion, gelatin/alginate scaffolds, with or without HAP, fabricated by 3D plotting according to a predesigned CAD-model might be potential candidates for the repair of bony and chondral defects, especially in complex defect situations affecting the osteochondral tissue interface since biphasic scaffolds with a stable connection of the two parts can be easily fabricated by multi-channel 3D plotting.

Graphical abstract: Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting

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Publication details

The article was received on 11 Mar 2015, accepted on 01 May 2015 and first published on 01 May 2015


Article type: Paper
DOI: 10.1039/C5RA04308E
Citation: RSC Adv., 2015,5, 43480-43488
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    Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting

    Y. Luo, A. Lode, A. R. Akkineni and M. Gelinsky, RSC Adv., 2015, 5, 43480
    DOI: 10.1039/C5RA04308E

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