Enrichment and purification of polyphenols in pine cone extracts of Pinus koraiensis Sieb. et Zucc. using a novel multi-channel parallel–serial chromatographic system packed with macroporous resin

Abstract

The present study uses a novel multi-channel parallel–serial chromatographic system packed with macroporous resin in the enrichment and purification of polyphenols in the pine cone extracts of Pinus koraiensis. The performance and separation characteristics of five macroporous resins have been evaluated, and the adsorption and desorption properties compared. According to the analysis results, AB-8 resin offers higher adsorption and desorption capacities than other resins. The experimental data are described by the pseudo second-order kinetic model and Langmuir and Freundlich isotherm. The multi-channel parallel–serial chromatographic system based on distinguishment between the loading zone and the separating zone packed with AB-8 resin is used to perform dynamic adsorption and desorption tests to optimize the enrichment process. The optimum conditions are: ethanol concentration, elute rate, sample volume and the concentration of polyphenols are 60% (v/v), 30 mL min⁻¹, 80 mL and 1.2 mg mL⁻¹, respectively. After one run treatment, the purity of polyphenols from SC-3 separation columns increased from 5.32% to 38.39%, 7.22 times to extracts; and its antioxidant capacity also enhanced significantly. The results show that the multi-channel parallel–serial chromatographic system reveals a high ability in the enrichment and purification of polyphenols.