Jump to main content
Jump to site search

Issue 51, 2015
Previous Article Next Article

Development and validation of a gas chromatography-mass spectrometry method for determination of sterol oxidation products in edible oils

Author affiliations

Abstract

An efficient gas chromatography-mass spectrometry method was developed and validated for determination of sterol oxidation products (SOPs) in edible oils. The sample preparation involved cold saponification, liquid–liquid extraction, solid-phase extraction on a silica gel cartridge, and trimethylsilylation by N-methyl-N-(trimethylsilyl) heptafluorobutyramide with 5% 1-methyl imidazole. The trimethylsilyl ether derivatives of SOPs were separated by gas chromatography with a 30 m DB-5MS capillary column and quantified by a mass spectrometer in selective ion monitoring mode. 5α-Cholestane and 19-hydroxycholesterol were used as dual internal standards. The calibration curves for each compound showed correlation coefficients (R2) better than 0.98. The detection limits were below 12.9 ng mL−1 (except for epoxides). The intra- and inter-day determination precisions for diversiform SOPs were <10% in relative standard deviations; the recoveries ranged within 89.72% and 117.42%. The developed approach was successfully applied to study the presence of thirty-four different SOPs present at low levels in camellia, olive, sesame, peanut, rapeseed, rice bran, soybean and corn oils.

Graphical abstract: Development and validation of a gas chromatography-mass spectrometry method for determination of sterol oxidation products in edible oils

Back to tab navigation

Supplementary files

Article information


Submitted
12 Feb 2015
Accepted
30 Apr 2015
First published
01 May 2015

RSC Adv., 2015,5, 41259-41268
Article type
Paper
Author version available

Development and validation of a gas chromatography-mass spectrometry method for determination of sterol oxidation products in edible oils

Y. Hu, G. Yang, W. Huang, S. Lai, Y. Ren, B. Huang, L. Zhang, P. Li and B. Lu, RSC Adv., 2015, 5, 41259
DOI: 10.1039/C5RA02795K

Social activity

Search articles by author

Spotlight

Advertisements