Issue 22, 2015

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

Abstract

Here we present a robust, stable and low-noise experimental set-up for performing electrochemical detection on a centrifugal microfluidic platform. By using a low-noise electronic component (electrical slip-ring) it is possible to achieve continuous, on-line monitoring of electrochemical experiments, even when the microfluidic disc is spinning at high velocities. Automated sample handling is achieved by designing a microfluidic system to release analyte sequentially, utilizing on-disc passive valving. In addition, the microfluidic system is designed to trap and keep the liquid sample stationary during analysis. In this way it is possible to perform cyclic voltammetry (CV) measurements at varying spin speeds, without altering the electrochemical response. This greatly simplifies the interpretation and quantification of data. Finally, real-time and continuous monitoring of an entire electrochemical experiment, including all intermediate sample handling steps, is demonstrated by amperometric detection of on-disc mixing of analytes (PBS and ferricyanide).

Graphical abstract: Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

Supplementary files

Article information

Article type
Paper
Submitted
22 Dec 2014
Accepted
29 Jan 2015
First published
30 Jan 2015

RSC Adv., 2015,5, 17187-17193

Integrating electrochemical detection with centrifugal microfluidics for real-time and fully automated sample testing

S. Z. Andreasen, D. Kwasny, L. Amato, A. L. Brøgger, F. G. Bosco, K. B. Andersen, W. E. Svendsen and A. Boisen, RSC Adv., 2015, 5, 17187 DOI: 10.1039/C4RA16858E

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