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Issue 20, 2015
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Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

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Abstract

Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL−1 to 10 μg mL−1 with a lower detection limit of 0.3 pg mL−1. The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards.

Graphical abstract: Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

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Supplementary files

Article information


Submitted
05 Dec 2014
Accepted
26 Jan 2015
First published
28 Jan 2015

RSC Adv., 2015,5, 15728-15735
Article type
Paper
Author version available

Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

J. Jin, J. Kim, T. Jeon, S. Shin, J. Sohn, H. Yi and B. Y. Lee, RSC Adv., 2015, 5, 15728
DOI: 10.1039/C4RA15815F

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