Box–Behnken designed adsorption based elution – unique separation process for commercially important acetyl shikonin from Arnebia nobilis

Abstract

Acetyl shikonin (AS), a pharmaceutically and commercially important compound, is a minor constituent in the industrial crop Arnebia nobilis. A Box–Behnken optimized, robust separation technique for AS was established by an adsorption based elution method. Optimized yields depict an increase of 78.9% total recovery of AS (97.8% pure), compared to conventional chromatography.