Issue 1, 2015

A tandem cell for nanopore-based DNA sequencing with exonuclease

Abstract

A tandem electrolytic cell with the structure [cis1, upstream nanopore (UNP), trans1 = cis2, downstream nanopore (DNP), trans2], an exonuclease enzyme attached to the downstream side of UNP, and a chemical adapter in or a profiled voltage over DNP can be used with bandwidths of a few kHz to sequence bases in ssDNA in natural order with high accuracy and without loss in trans1/cis2 or regression into DNP from trans2.

Graphical abstract: A tandem cell for nanopore-based DNA sequencing with exonuclease

Supplementary files

Article information

Article type
Communication
Submitted
12 Sep 2014
Accepted
17 Oct 2014
First published
17 Oct 2014

RSC Adv., 2015,5, 167-171

Author version available

A tandem cell for nanopore-based DNA sequencing with exonuclease

G. Sampath, RSC Adv., 2015, 5, 167 DOI: 10.1039/C4RA10326B

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