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Issue 39, 2015
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A gold immunochromatographic assay for the rapid and simultaneous detection of fifteen β-lactams

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Abstract

A novel gold immunochromatographic assay (GICA) based on anti-β-lactam receptors was innovatively developed that successfully allowed rapid and simultaneous detection of fifteen β-lactams in milk samples in 5–10 minutes. By replacing the antibodies used in traditional GICA with anti-β-lactam receptors, the difficulty in producing broad specific antibodies against β-lactams was overcome. Conjugates of ampicillin with BSA and goat anti-mouse immunoglobulin (IgG) were immobilized onto the test and control lines on the nitrocellulose membrane, respectively. Since goat anti-mouse IgG does not combine with receptors, negative serum from mice labelled with gold nanoparticles (GNP) was mixed with GNP-labelled receptors. Results were obtained within 20 min using a paper-based sensor. The utility of the assay was confirmed by the analysis of milk samples. The limits of detection (LOD) for amoxicillin, ampicillin, penicillin G, penicillin V, cloxacillin, dicloxacillin, nafcillin, oxacillin, cefaclor, ceftezole, cefotaxime, ceftiofur, cefoperazone, cefathiamidine, and cefepime were 0.25, 0.5, 0.5, 0.5, 1, 5, 5, 10, 25, 10, 100, 10, 5, 5, and 2 ng mL−1, respectively, which satisfies the maximum residue limits (MRL) set by the European Union (EU). In conclusion, our newly developed GICA-based anti-β-lactam receptor assay provides a rapid and effective method for one-site detection of multiple β-lactams in milk samples.

Graphical abstract: A gold immunochromatographic assay for the rapid and simultaneous detection of fifteen β-lactams

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Publication details

The article was received on 25 Jul 2015, accepted on 29 Aug 2015 and first published on 08 Sep 2015


Article type: Paper
DOI: 10.1039/C5NR04987C
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Nanoscale, 2015,7, 16381-16388

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    A gold immunochromatographic assay for the rapid and simultaneous detection of fifteen β-lactams

    Y. Chen, Y. Wang, L. Liu, X. Wu, L. Xu, H. Kuang, A. Li and C. Xu, Nanoscale, 2015, 7, 16381
    DOI: 10.1039/C5NR04987C

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