Issue 34, 2015

Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization

Abstract

A facile method to prepare a biocompatible graphene oxide (GO)-based substrate for protein immobilization was developed to overcome the drawbacks of GO, such as the strong electrostatic and hydrophobic interactions which could potentially alter the conformation and biological activity of proteins. The GO was coated with hydrophilic branched polyethyleneimine (BPEI), while Concanavalin A (Con A) as a model lectin protein was employed to fabricate the functionalized composites to evaluate the feasibility of this strategy. The composites exhibit an extremely high binding capacity for glycoproteins (i.e. IgG 538.3 mg g−1), which are superior to other immobilized materials. Moreover, they can work well in 500-fold non-glycoprotein interference and even in complex biological samples. All these data suggest that the GO@BPEI composites will have great potential as scaffolds for proteins fully exerting their biofunctions.

Graphical abstract: Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization

Supplementary files

Article information

Article type
Paper
Submitted
22 May 2015
Accepted
17 Jul 2015
First published
22 Jul 2015

Nanoscale, 2015,7, 14284-14291

Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization

Y. Weng, B. Jiang, K. Yang, Z. Sui, L. Zhang and Y. Zhang, Nanoscale, 2015, 7, 14284 DOI: 10.1039/C5NR03370E

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