Issue 13, 2015

Graphene oxide immobilized enzymes show high thermal and solvent stability

Abstract

The thermal and solvent tolerance of enzymes is highly important for their industrial use. We show here that the enzyme lipase from Rhizopus oryzae exhibits exceptionally high thermal stability and high solvent tolerance and even increased activity in acetone when immobilized onto a graphene oxide (GO) nanosupport prepared by Staudenmaier and Brodie methods. We studied various forms of immobilization of the enzyme: by physical adsorption, covalent attachment, and additional crosslinking. The activity recovery was shown to be dependent on the support type, enzyme loading and immobilization procedure. Covalently immobilized lipase showed significantly better resistance to heat inactivation (the activity recovery was 65% at 70 °C) in comparison with the soluble counterpart (the activity recovery was 65% at 40 °C). Physically adsorbed lipase achieved over 100% of the initial activity in a series of organic solvents. These findings, showing enhanced thermal stability and solvent tolerance of graphene oxide immobilized enzyme, will have a profound impact on practical industrial scale uses of enzymes for the conversion of lipids into fuels.

Graphical abstract: Graphene oxide immobilized enzymes show high thermal and solvent stability

Supplementary files

Article information

Article type
Paper
Submitted
21 Jan 2015
Accepted
18 Feb 2015
First published
25 Feb 2015
This article is Open Access
Creative Commons BY license

Nanoscale, 2015,7, 5852-5858

Author version available

Graphene oxide immobilized enzymes show high thermal and solvent stability

S. Hermanová, M. Zarevúcká, D. Bouša, M. Pumera and Z. Sofer, Nanoscale, 2015, 7, 5852 DOI: 10.1039/C5NR00438A

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