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Issue 9, 2015
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Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells: improving bioanalysis using electrochemistry

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Abstract

There is a great demand to develop novel techniques that allow useful and complete monitoring of apoptosis, which is a key factor of several diseases and a target for drug development. Here, we present the use of a novel dual electrochemical/optical label for the detection and study of apoptosis. We combined the specificity of Annexin-V for phosphatidylserine, a phospholipid expressed in the outer membrane of apoptotic cells, with the optical and electrochemical properties of quantum dots to create a more efficient label. Using this conjugate we addressed three important issues: (i) we made the labeling of apoptotic cells faster (30 min) and easier; (ii) we fully characterized the samples by common cell biological techniques (confocal laser scanning microscopy, scanning electron microscopy and flow cytometry); and (iii) we developed a fast, cheap and quantitative electrochemical detection method for apoptotic cells with results in full agreement with those obtained by flow cytometry.

Graphical abstract: Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells: improving bioanalysis using electrochemistry

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Publication details

The article was received on 05 Dec 2014, accepted on 27 Jan 2015 and first published on 29 Jan 2015


Article type: Paper
DOI: 10.1039/C4NR07191C
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Citation: Nanoscale, 2015,7, 4097-4104
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    Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells: improving bioanalysis using electrochemistry

    H. Montón, C. Parolo, A. Aranda-Ramos, A. Merkoçi and C. Nogués, Nanoscale, 2015, 7, 4097
    DOI: 10.1039/C4NR07191C

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