Issue 6, 2015

Electron transfer and fluorescence “turn-off” based CdTe quantum dots for vancomycin detection at nanogram level in aqueous serum media

Abstract

A simple and sensitive fluorescence “turn-off” biosensor for detection of vancomycin at nanogram level was proposed based on the electron transfer mechanism and the fluorescence quenching of CdTe quantum dots (QDs). The electron transfer process during the interaction between vancomycin and GSH (glutathione)-CdTe QDs was investigated not only by ultraviolet/visible (UV/vis) absorption and fluorescence (FL) spectroscopy but also by fluorescence lifetime measurements. The degree of the electron transfer and as resulted fluorescence quenching was proportional to the increasing of vancomycin concentration in the range of 1.534 ng mL−1–20 μg mL−1, with a corresponding detection limit of 0.4605 ng mL−1. This proposed a biosensor that could be used to determine vancomycin in environmental water samples, pharmaceutical formulation and spiked human serum with all of the recoveries over 95.8%. The mechanism of the detection was dynamic quenching with an electron transfer (ET) process. The experimental conditions, key affecting factors and the influence of the coexisting substances have also been optimized and studied.

Graphical abstract: Electron transfer and fluorescence “turn-off” based CdTe quantum dots for vancomycin detection at nanogram level in aqueous serum media

Supplementary files

Article information

Article type
Paper
Submitted
09 Oct 2014
Accepted
30 Mar 2015
First published
30 Mar 2015

New J. Chem., 2015,39, 4774-4782

Author version available

Electron transfer and fluorescence “turn-off” based CdTe quantum dots for vancomycin detection at nanogram level in aqueous serum media

W. Liang, S. Liu, Z. Liu, D. Li, L. Wang, C. Hao and Y. He, New J. Chem., 2015, 39, 4774 DOI: 10.1039/C4NJ01764A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements