Jump to main content
Jump to site search

Issue 2, 2015
Previous Article Next Article

Antimicrobial spine-bone cement with caffeic acid phenethyl ester for controlled release formulation and in vivo biological assessments

Author affiliations

Abstract

The work reports the preparation of antimicrobial spine-bone-cement with a highly controlled release formulation that was subjected to tests, involving in vitro and in vivo biological assessments such as antimicrobial effects, a cytotoxicity test, a bacterial reverse mutation (Ames) assay, a micronucleus assay, and implantation analysis. Two kinds of acrylic bone-cements were tested for the expression of antimicrobial and antibiotic activity. For these tests, new antimicrobial bone-cement was prepared using a caffeic acid phenethyl ester (CAPE) compound from natural propolis, and an antibiotic bone-cement was prepared by the inclusion of gentamycin (GM). The antimicrobial ability and controlled release property of both CAPE-loaded and GM-loaded bone-cements were demonstrated as a function of time, concentration and optimization to maximize their antimicrobial effects against Staphylococcus aureus bacteria. The compressive strengths of both bone-cements, at various CAPE and GM concentrations, were over 130 MPa and 80 MPa for CAPE-and GM-loaded bone-cement, respectively. Moreover, the CAPE-loaded antimicrobial bone-cements showed a controlled release pattern better than that of the GM-loaded antibiotic bone-cement due to their homogeneous loading. Through various in vitro and in vivo biological assessments, the CAPE-loaded antimicrobial bone-cements showed positive results in all assessments, without any inflammatory response.

Graphical abstract: Antimicrobial spine-bone cement with caffeic acid phenethyl ester for controlled release formulation and in vivo biological assessments

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Jun 2014, accepted on 31 Oct 2014 and first published on 03 Nov 2014


Article type: Concise Article
DOI: 10.1039/C4MD00272E
Author version
available:
Download author version (PDF)
Med. Chem. Commun., 2015,6, 327-333

  •   Request permissions

    Antimicrobial spine-bone cement with caffeic acid phenethyl ester for controlled release formulation and in vivo biological assessments

    H. S. Lee and J. H. Chang, Med. Chem. Commun., 2015, 6, 327
    DOI: 10.1039/C4MD00272E

Search articles by author

Spotlight

Advertisements