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Issue 11, 2015
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Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and “antigen surrogates” mounted onto a liquid array

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Abstract

Autoantibodies raised against β cell antigens are the most reliable preclinical biomarkers for predicting the imminent onset of type 1 diabetes mellitus (T1DM). The most current detection platforms are technically challenging or are run on clinically esoteric equipment. Here, we present a straightforward approach to detect autoantibody biomarkers that employs highly PEGylated microspheres onto which are mounted various capture agents that include affinity-tagged antigens or small molecule “antigen surrogates.” After incubation with small quantities of serum, the bound autoantibodies can be measured using a standard flow cytometer. By multiplexing this assay, we show that a panel of antigen and antigen surrogates reliably predicts hyperglycemia in a mouse model of diabetes without false positives.

Graphical abstract: Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and “antigen surrogates” mounted onto a liquid array

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Supplementary files

Article information


Submitted
01 Aug 2015
Accepted
14 Sep 2015
First published
22 Sep 2015

Mol. BioSyst., 2015,11, 3156-3163
Article type
Paper
Author version available

Reliable diagnosis of murine type 1 diabetes using a panel of autoantigens and “antigen surrogates” mounted onto a liquid array

T. M. Doran, J. Morimoto, S. Simanski, P. J. McEnaney and T. Kodadek, Mol. BioSyst., 2015, 11, 3156
DOI: 10.1039/C5MB00521C

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