Jump to main content
Jump to site search

Issue 3, 2015
Previous Article Next Article

Metabolomic analysis reveals functional overlapping of three signal transduction proteins in regulating ethanol tolerance in cyanobacterium Synechocystis sp. PCC 6803

Author affiliations

Abstract

Low ethanol tolerance is a crucial factor that restricts the feasibility of bioethanol production in renewable cyanobacterial systems. Our previous studies showed that several transcriptional regulators were differentially regulated by exogenous ethanol in Synechocystis. In this study, by constructing knockout mutants of 34 Synechocystis putative transcriptional regulator-encoding genes and analyzing their phenotypes under ethanol stress, we found that three mutants of regulatory gene sll1392, sll1712 and slr1860 grew poorly in the BG11 medium supplemented with ethanol when compared with the wild type in the same medium, suggesting that the genes may be involved in the regulation of ethanol tolerance. To decipher the regulatory mechanism, targeted LC-MS and untargeted GC-MS approaches were employed to determine metabolic profiles of the three mutants and the wild type under both normal and ethanol stress conditions. The results were then subjected to PCA and WGCNA analyses to determine the responsive metabolites and metabolic modules related to ethanol tolerance. Interestingly, the results showed that there was a significant overlapping of the responsive metabolites and metabolic modules between three regulatory proteins, suggesting that a possible crosstalk between various regulatory proteins may be involved in combating against ethanol toxicity in Synechocystis. The study provided new insights into ethanol-tolerance regulation and knowledge important to rational tolerance engineering in Synechocystis.

Graphical abstract: Metabolomic analysis reveals functional overlapping of three signal transduction proteins in regulating ethanol tolerance in cyanobacterium Synechocystis sp. PCC 6803

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Nov 2014, accepted on 01 Dec 2014 and first published on 05 Dec 2014


Article type: Paper
DOI: 10.1039/C4MB00651H
Author version
available:
Download author version (PDF)
Mol. BioSyst., 2015,11, 770-782

  •   Request permissions

    Metabolomic analysis reveals functional overlapping of three signal transduction proteins in regulating ethanol tolerance in cyanobacterium Synechocystis sp. PCC 6803

    Y. Zhu, G. Pei, X. Niu, M. Shi, M. Zhang, L. Chen and W. Zhang, Mol. BioSyst., 2015, 11, 770
    DOI: 10.1039/C4MB00651H

Search articles by author

Spotlight

Advertisements