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Issue 2, 2015
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High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

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Abstract

Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.

Graphical abstract: High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

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Article information


Submitted
20 Sep 2014
Accepted
18 Nov 2014
First published
18 Nov 2014

Mol. BioSyst., 2015,11, 361-365
Article type
Communication

High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

N. Takemori, A. Takemori, K. Matsuoka, R. Morishita, N. Matsushita, M. Aoshima, H. Takeda, T. Sawasaki, Y. Endo and S. Higashiyama, Mol. BioSyst., 2015, 11, 361
DOI: 10.1039/C4MB00556B

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