Jump to main content
Jump to site search

Issue 19, 2015
Previous Article Next Article

A high-throughput acoustic cell sorter

Author affiliations


Acoustic-based fluorescence activated cell sorters (FACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. However, the sorting throughput for existing acoustic cell sorters is far from optimum for practical applications. Here we report a high-throughput cell sorting method based on standing surface acoustic waves (SSAWs). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. As a result, the sorting throughput is improved significantly from conventional acoustic-based cell sorting methods. We demonstrated the successful sorting of 10 μm polystyrene particles with a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13 800 events per second. Without using a cell-detection unit, we were able to demonstrate an actual sorting throughput of 3300 events per second. Our sorting method can be conveniently integrated with upstream detection units, and it represents an important development towards a functional acoustic-based FACS system.

Graphical abstract: A high-throughput acoustic cell sorter

Back to tab navigation

Supplementary files

Article information

23 Jun 2015
04 Aug 2015
First published
20 Aug 2015

Lab Chip, 2015,15, 3870-3879
Article type
Author version available

A high-throughput acoustic cell sorter

L. Ren, Y. Chen, P. Li, Z. Mao, P. Huang, J. Rufo, F. Guo, L. Wang, J. P. McCoy, S. J. Levine and T. J. Huang, Lab Chip, 2015, 15, 3870
DOI: 10.1039/C5LC00706B

Social activity

Search articles by author