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Issue 18, 2015
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A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays

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Abstract

Nanostructured microelectrodes (NMEs) are three-dimensional electrodes that have superb sensitivity for electroanalysis. Here we report the integration of NMEs with the versatile fluid-handling system digital microfluidics (DMF), for eventual application to distributed diagnostics outside of the laboratory. In the new methods reported here, indium tin oxide DMF top plates were modified to include Au NMEs as well as counter and pseudoreference electrodes. The new system was observed to outperform planar sensing electrodes of the type that are typically integrated with DMF. A rubella virus (RV) IgG immunoassay was developed to evaluate the diagnostic potential for the new system, relying on magnetic microparticles coated with RV particles and analysis by differential pulse voltammetry. The limit of detection of the assay (0.07 IU mL−1) was >100× below the World Health Organization defined cut-off for rubella immunity. The sensitivity of the integrated device and its small size suggest future utility for distributed diagnostics.

Graphical abstract: A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays

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Publication details

The article was received on 14 Jun 2015, accepted on 24 Jul 2015 and first published on 06 Aug 2015


Article type: Paper
DOI: 10.1039/C5LC00660K
Citation: Lab Chip, 2015,15, 3776-3784
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    A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays

    D. G. Rackus, M. D. M. Dryden, J. Lamanna, A. Zaragoza, B. Lam, S. O. Kelley and A. R. Wheeler, Lab Chip, 2015, 15, 3776
    DOI: 10.1039/C5LC00660K

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