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Issue 10, 2015
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Fabrication and characterization of optogenetic, multi-strip cardiac muscles

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Cardiac tissue engineering aims to recreate functional tissue constructs similar to the structure and function of the native myocardium. To date, in vitro tissue constructs lack the architectural complexity of a vascular network and the precise motor unit control of muscle fibers. Here, we present a method to construct engineered multi-strip cardiac muscle that simulates the bundle-like architecture of the native myocardium. Densely packed primary myocytes and cardiac fibroblasts were co-cultured with optogenetic, non-excitable cells. The resulting 3D syncytium triggered contraction upon localized blue light illumination to selectively activate and pace the multi-strip cardiac muscles, similar to the activity of pacemaker cells. Acting on a single load, we demonstrated graded force production through light-modulated multi-strip recruitment. These results demonstrate an in vitro platform of optogenetic, multi-strip cardiac muscles that can be used in a wide variety of applications, such as drug discovery, tissue engineering, and bio-hybrid robotic systems.

Graphical abstract: Fabrication and characterization of optogenetic, multi-strip cardiac muscles

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The article was received on 21 Feb 2015, accepted on 01 Apr 2015 and first published on 02 Apr 2015

Article type: Paper
DOI: 10.1039/C5LC00222B
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Lab Chip, 2015,15, 2258-2268

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    Fabrication and characterization of optogenetic, multi-strip cardiac muscles

    V. Chan, D. M. Neal, S. G. M. Uzel, H. Kim, R. Bashir and H. H. Asada, Lab Chip, 2015, 15, 2258
    DOI: 10.1039/C5LC00222B

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