Jump to main content
Jump to site search

Issue 6, 2015
Previous Article Next Article

HIV microarray for the mapping and characterization of HIV-specific antibody responses

Author affiliations

Abstract

We used the microarray technology to develop chips containing a comprehensive set of proteins and peptides covering the proteome of HIV-1 clade C, which is the HIV-1 subtype that causes the majority of infections worldwide. We demonstrate that the HIV microarray allows simultaneous, sensitive and specific detection of antibody responses for the major immunoglobulin classes (IgG, IgA, IgM, IgE) and subclasses (IgG1–4) with minute amounts of serum samples towards a large number of HIV antigens and peptides. Furthermore, we show that the HIV chip can be used for the monitoring of antibody responses during the course of the disease and during treatment. The HIV microarray should be useful to study antibody responses to multiple HIV antigens and epitopes in HIV-infected patients to explore pathomechanisms of the disease, for diagnosis and for monitoring of treatment and of vaccine trials.

Graphical abstract: HIV microarray for the mapping and characterization of HIV-specific antibody responses

Back to tab navigation

Supplementary files

Article information


Submitted
29 Dec 2014
Accepted
19 Jan 2015
First published
19 Jan 2015

This article is Open Access

Lab Chip, 2015,15, 1574-1589
Article type
Paper
Author version available

HIV microarray for the mapping and characterization of HIV-specific antibody responses

D. Gallerano, E. Wollmann, C. Lupinek, T. Schlederer, D. Ebner, C. Harwanegg, K. Niespodziana, K. Schmetterer, W. Pickl, E. Puchhammer-Stöckl, E. Sibanda and R. Valenta, Lab Chip, 2015, 15, 1574
DOI: 10.1039/C4LC01510J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements