Jump to main content
Jump to site search

Issue 8, 2015
Previous Article Next Article

Insights into the effect of dilute acid, hot water or alkaline pretreatment on the cellulose accessible surface area and the overall porosity of Populus

Author affiliations

Abstract

Pretreatment is known to make biomass more reactive to cellulase by altering the chemical compositions as well as physical structures of biomass. Simons’ staining technique along with mercury porosimetry was applied on the acid, neutral, and alkaline pretreated materials to measure the accessible surface area of cellulose and pore size distribution of Populus. The results indicated that acid pretreatment is much more effective than water and alkaline pretreatment in terms of cellulose accessibility increase. Further investigation suggests that lignin does not dictate cellulose accessibility to the extent that hemicellulose does, but it does restrict xylan accessibility which in turn controls the access of cellulase to cellulose. The most interesting finding is that severe acid pretreatment significantly decreases the average pore size, i.e. 90% average size decrease could be observed after 60 min dilute acid pretreatment at 160 °C; however, the nano-pore space formed between the coated microfibrils increased after pretreatment, especially with the acid pretreatment, suggesting that this particular type of biomass porosity is probably the most fundamental barrier to effective enzymatic hydrolysis.

Graphical abstract: Insights into the effect of dilute acid, hot water or alkaline pretreatment on the cellulose accessible surface area and the overall porosity of Populus

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 Mar 2015, accepted on 29 May 2015 and first published on 19 Jun 2015


Article type: Paper
DOI: 10.1039/C5GC00689A
Citation: Green Chem., 2015,17, 4239-4246
  •   Request permissions

    Insights into the effect of dilute acid, hot water or alkaline pretreatment on the cellulose accessible surface area and the overall porosity of Populus

    X. Meng, T. Wells, Q. Sun, F. Huang and A. Ragauskas, Green Chem., 2015, 17, 4239
    DOI: 10.1039/C5GC00689A

Search articles by author

Spotlight

Advertisements