Jump to main content
Jump to site search

Issue 11, 2015
Previous Article Next Article

Metabolomics strategy reveals therapeutical assessment of limonin on nonbacterial prostatitis

Author affiliations

Abstract

Limonin has been found to possess significant anti-inflammatory properties in animal tests and with, human cells, however, its precise metabolism mechanism has not been well explored. The aim of this study was to investigate the anti-inflammatory effects of limonin in a nonbacterial prostatitis (NBP) animal model. Global metabolite profiling was performed by ultra-high-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC/ESI-TOFMS) and in conjunction with multivariate data analysis and pathway analysis which were integrated to explore differentiating metabolites and clarify the mechanism of limonin against capsaicin-induced NBP. Limonin has a potential protective function revealed by the metabolic profiling of limonin-treated rats located closer to the normal group. Twenty potential biomarker candidates and several key metabolic pathways contributing to the treatment of NBP were discovered and identified. Among the pathways, the related glycine, serine and threonine metabolism, glycerophospholipid metabolism were acutely perturbed. The changes in metabolites were restored to their base-line levels after limonin treatment, which might be through regulating the perturbed pathways to the normal state. The results indicate that changed biomarkers and pathways may provide evidence and insight into limonin action mechanisms and enable us to increase research productivity toward metabolomics in therapeutical assessment and drug discovery.

Graphical abstract: Metabolomics strategy reveals therapeutical assessment of limonin on nonbacterial prostatitis

Back to tab navigation

Supplementary files

Article information


Submitted
03 May 2015
Accepted
05 Aug 2015
First published
07 Aug 2015

Food Funct., 2015,6, 3540-3549
Article type
Paper
Author version available

Metabolomics strategy reveals therapeutical assessment of limonin on nonbacterial prostatitis

A. Zhang, H. Wang, H. Sun, Y. Zhang, N. An, G. Yan, X. Meng and X. Wang, Food Funct., 2015, 6, 3540
DOI: 10.1039/C5FO00489F

Social activity

Search articles by author

Spotlight

Advertisements